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In Vitro Mitogenic Stimulation of Murine Spleen Cells by Herpes Simplex Virus

Department of Virology, German Cancer Research Center, Heidelberg, and Institute of Medical Virology, University of Heidelberg Heidelberg, West Germany

Abstract

Spleen cells of B6 mice not previously immunized were induced to DNA synthesis by supernatants from HSV-infected tissue culture. The stimulatory principle could be passed through a 45-µm filter and sedimented at 100,000 x G. It was abolished by UV light, heating at 56°C, and by an anti-HSV serum. The possibility that the observed stimulation was caused by LPS was therefore excluded, and there was also no indication of mycoplasma contamination. Partial purification of spleen cells from macrophages resulted in an increased stimulation by HSV. From experiments with nylon columns, anti- antibody, and nude mice it was concluded that HSV acted as a B cell mitogen. Strains of both HSV types 1 and 2 were stimulatory for B6 spleen cells. Of nine freshly isolated HSV strains with identical passage history (twice in HEF) four were strongly stimulatory, three showed a moderate stimulation, and two did not stimulate. Spleen cells from A/J and DBA/2 mice were stimulated to the same extent by HSV (WAL) as spleen cells from B6 mice. No viral replication was demonstrable in B6 spleen cell cultures stimulated for DNA synthesis by HSV. Thus our study demonstrates induction of cellular DNA synthesis in B lymphocytes by HSV which is abolished by inactivation of the virus.

Footnotes

¹ Please address correspondence to H. Kirchner, Institut für Virusforschung, Deutsches Krebsforschungszentrum, In Neuenheimer Feld 280, 6900 Heidelberg, FRG.

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