The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1978, 120: 513-519.
Copyright © 1978 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tada, N.
Right arrow Articles by Pressman, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Tada, N.
Right arrow Articles by Pressman, D.

Human Cell Membrane Components Bound to beta2-Microglobulin in T Cell-Type Cell Lines1

Nobuhiko Tada2, Nobuyuki Tanigaki and David Pressman

Department of Immunology Research, Roswell Park Memorial Institute, 3 666 Elm Street, Buffalo, New York 14263

Abstract

Cell membrane components bound to beta2-microglobulin were isolated from Renex 30 (a nonionic detergent)-solubilized membrane materials of two human T cell-type cell lines, MOLT-4 and CCRF-CEM, by gel filtration and lectin affinity chromatography. The isolation was carried out by following the beta2-microglobulin activity by radioimmune inhibition assay. The T cell membrane components bound to beta2-microglobulin had a uniform molecular size of about 200,000 daltons and most of them showed an affinity to lentil lectin. The isolated membrane components were radioiodinated and examined for identity to HLA antigens by sequential precipitation with rabbit anti-HLA antiserum (specific to HLA large components) and with rabbit anti-beta2-microglobulin antiserum. In addition to HLA antigens, the beta2-microglobulin-bound components obtained from the MOLT-4 cells were found to contain certain membrane components that are the same in molecular size as the HLA large components but that are different antigenically from the HLA large components. On the other hand, the beta2-microglobulin-bound membrane components obtained from the CCRF-CEM cells were all HLA antigens. No other membrane components were involved in the binding.

Footnotes

1 This work was supported by Public Health Service Research Grants AI-08899, CA-17276, and CA-17609.

2 On leave of absence from Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.

3 A unit of the New York State Department of Health.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1978 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1978 by The American Association of Immunologists, Inc. All rights reserved.