HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Yamamura, Y. Search for Related Content PubMed Articles by Yamamura, Y.

Immunologic Responses to a Murine Mammary Adenocarcinoma: in Vitro Production of Specific Killer Cells Is Dependent on Active T Lymphocytes¹

Clinical Radiation Therapy Research Center, Cancer Research Unit, Allegheny General Hospital, 320 East North Avenue, Pittsburgh, Pennsylvania 15212

Abstract

The mode of production of specifically armed monocytic killer cells was investigated with the T1699 mammary adenocarcinoma in syngeneic DBA/2 mice. After overnight in vitro incubation of cells from the spleen but not from the lymph nodes, blood, or from the peritoneal cavity produced specific killer cells. The activation of spleen cells was inhibited by pretreatment with anti- serum and C; however, already activated specific killer cells were not sensitive to the same treatment. Removal of phagocytic cells did not significantly affect the cytotoxicity of the splenic killer cells whereas removal of rayon-wool adherent cells greatly reduced both the total cytotoxicity, and to a lesser extent, the cytotoxicity indices. Overnight co-cultivation of normal peritoneal-exudate cells with the lymph node cells from tumor-bearers, although neither class of cells alone was cytotoxic to T1699 cells in vitro, produced specific monocytic killer cells, through steps dependent on active T lymphocyte functions. Culture supernatants of tumor-bearer's spleen cells also contained factor(s) which induced cytotoxicity mediated by normal peritoneal-exudate cells against T1699 cells in vitro; and the production of the factor(s) was also inhibited by pretreatment of the spleen cells with anti- serum but not by anti-mouse IgG or anti-mouse whole -globulins serum and C.

Footnotes

¹ This work was supported in part by United States Public Health Service Grant CA-10438-11, ACS Grant IM-84, and South Carolina Appropriation for Biomedical Research.