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Alterations in Intramembrane Particle Distribution during Interaction of Erythrocyte-Bound Ligands with Immunoprotein Receptors¹

Departments of Medicine and Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455

Abstract

Freeze fracture studies have been performed on rabbit pulmonary alveolar macrophages and a nonphagocytic murine lymphoblastoid cell line, PU-5 Fc+, incubated with sheep erythrocytes, sheep erythrocyte-IgG Forssman antibody complex, sheep erythrocyte-IgG Forssman antibody-C complexes and aggregated IgG. Alveolar macrophages show redistribution of intramembrane particles after interaction with E(IgG) and E(IgM)C. The murine lymphoblastoid cell line shows intramembrane particle redistribution consequential to binding of E(IgG) and aggregated IgG. The results demonstrate that after specific immunoprotein receptor-ligand interaction, there is extensive plasma membrane reorganization which results in a redistribution and loss of intramembrane particles. Changes are observed in the protoplasmic face of the plasma membrane after the binding of ligand to the outer membrane surface. The findings suggest that interaction of erythrocyte-bound ligands with specific lymphoid and macrophage plasma membrane receptors leads to a generalized redistribution of integral membrane components in the membrane.

Footnotes

¹ This work was supported by the National Institutes of Allergy and Infectious Diseases, United States Public Health Service Grant AI 12478, and by grants from the National Leukemia Association and the Kroc Foundation, Santa Ynez, California. This work was presented in part at the sixty-first Annual Meeting of the Federation of American Societies for Experimental Biology; Federation Proceedings 36:1314. 1977.