The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1977, 119: 1525-1529.
Copyright © 1977 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ferrarini, M.
Right arrow Articles by Kunkel, H. G.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Ferrarini, M.
Right arrow Articles by Kunkel, H. G.

Receptors for IgM on Certain Human B Lymphocytes1

Manlio Ferrarini2, Thomas Hoffman, Shu Man Fu, Robert Winchester and Henry G. Kunkel

The Rockefeller University, 1230 York Avenue, New York, N. Y. 10021

Abstract

A receptor for IgM was demonstrated on the surface of human B lymphocytes by using a rosette technique with ox erythrocytes coated with rabbit IgM antibody (EAM). Lymphocytes forming rosettes with EAM did not bind sheep red cells, had membrane Ia-like antigens and, in some instances, surface immunoglobulin. The specificity of EAM rosettes was confirmed by inhibition experiments with purified human Ig. IgM but not IgG molecules inhibited the rosette reaction. In addition, inhibition of EAM rosettes with IgM fragments showed that the receptor has affinity for a part of the molecule located in the Fc portion. By analogy with the receptors previously found on certain human T cells, receptors for IgM were not detected on freshly isolated B cells, but were expressed after overnight culture in IgM-free media. Studies on different human lymphoid tissues showed that IgM receptors are expressed on a limited percentage of both circulating and noncirculating B cells. In addition to normal B cells, the malignant B cells of a majority of cases of chronic lymphocytic leukemia expressed the receptors for IgM.

Footnotes

1 This work was supported by the National Foundation of the March of Dimes, National Institutes of Health Grant RR-102, and United States Public Health Service Grants AI 10811 and CA 20107.

2 On leave from the University of Genoa, Genoa, Italy. Recipient of a J. E. Fogarty Fellowship from the National Institutes of Health.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1977 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1977 by The American Association of Immunologists, Inc. All rights reserved.