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Antigenic Requirements for the Generation of Secondary Cytotoxicity¹

Immunobiology Research Center and Departments of Medical Genetics and Surgery, The University of Wisconsin, Madison, Wisconsin 53706

Abstract

The in vitro role of products of the H-2 complex in restimulation of secondary proliferative and cytotoxic responses has been studied. In this paper we have studied in particular the role of CD antigens, the target antigens for cytotoxic T lymphocytes. We have used UV-treated cells that express their CD antigens but no LD. Like others, we demonstrate that CD antigens alone are able to generate a secondary cytotoxic response and furthermore that these CD antigens must be the same as those used for primary sensitization. However, in contrast to the results of others, in some cases UV-treated cells were unable to restimulate such a response even when appropriate controls showed that the CD antigens on UV-treated cells were functional. Furthermore, in these cases the addition of an LD stimulus in the presence of UV-treated cells results in a secondary cytotoxic response that is significantly greater than that elicited by LD restimulation alone. Such potentiation is not observed if the CD antigens used for restimulation are different from those used for the primary sensitization. In addition, a non-H-2 differences(s), presumably the Mls locus, appears to be able to generate a secondary response. The implications of these results are discussed in terms of "memory" T lymphocytes in cell-mediated immunity.

Footnotes

¹ This work was supported in part by National Institutes of Health Grants CA-16836, AI-11576, and AI-08439, and National Foundation March of Dimes Grants CRBS 246 and 6-76-213. This is Paper No. 2105 from the Laboratory of Genetics and Paper No. 120 from the Immunobiology Research Center, The University of Wisconsin, Madison, Wisconsin 53706.

² C. G.-C. is an honorary fellow of the Délégation Générale de la Recherche Scientifique et Technique.