HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stamper, H. B. Articles by Woodruff, J. J. Search for Related Content PubMed Articles by Stamper, H. B., Jr. Articles by Woodruff, J. J.

An in vitro Model of Lymphocyte Homing

I. Characterization of the Interaction Between Thoracic Duct Lymphocytes and Specialized High-Endothelial Venules of Lymph Nodes¹

From the Department of Microbiology and Immunology, State University of New York, Downstate Medical Center, Brooklyn, New York 11203

Abstract

An in vitro system was used to study lymphocyte interaction with high-endothelial venules (HEV) in rat lymph nodes. The results show that thoracic duct lymphocytes (TDL) are capable of selective adherence to the endothelium of HEV when overlaid onto glutaraldehyde-fixed sections of lymph nodes. At optimal TDL concentration (30 x 10⁶/ml) and incubation temperature (7°C) binding occurs rapidly and the majority of HEV in each section usually shows adherent lymphocytes, the number attaching to each vessel being proportional to the endothelial area. TDL do not bind to other vascular structures in the node and ordinarily there is little adherence to other cells in the section. The results indicate therefore that TDL have surface membrane structures specific for HEV recognition.

Although TDL adhere to glutaraldehyde-fixed HEV, lymphocytes pretreated with fixative lose their capacity to bind. Also incubation performed at 1°C produces a fairly marked reduction in binding capacity of normal TDL. These observations indicate that endothelium of HEV are passive participants in the in vitro reaction whereas metabolic activity is important on the part of lymphocytes. In addition, when incubation is performed at 24°C, binding is severely reduced and at 37°C few, if any, HEV exhibit adherent TDL. It is suggested that lymphocytes that adhere to HEV are capable of rapid dissociation under physiologic conditions, a property that could facilitate entry of lymphocytes into the lymph node after their interaction with the specialized endothelium in vivo.

Footnotes

¹ This work was supported by Grant AI 10080 from the National Institutes of Health.

This article has been cited by other articles:

				N. Sharon and H. Lis History of lectins: from hemagglutinins to biological recognition molecules Glycobiology, November 1, 2004; 14(11): 53R - 62R. [Abstract] [Full Text] [PDF]

				U. G. Strauch, R. C. Mueller, X. Y. Li, M. Cernadas, J. M. G. Higgins, D. G. Binion, and C. M. Parker Integrin {{alpha}}E(CD103){{beta}}7 Mediates Adhesion to Intestinal Microvascular Endothelial Cell Lines Via an E-Cadherin-Independent Interaction J. Immunol., March 1, 2001; 166(5): 3506 - 3514. [Abstract] [Full Text] [PDF]

				G. M. Tanneau, L. H.-S. Oyant, C. C. Chevaleyre, and H. P. Salmon Differential Recruitment of T- and IgA B-lymphocytes in the Developing Mammary Gland in Relation to Homing Receptors and Vascular Addressins J. Histochem. Cytochem., December 1, 1999; 47(12): 1581 - 1592. [Abstract] [Full Text]

				K. Chiba, Y. Yanagawa, Y. Masubuchi, H. Kataoka, T. Kawaguchi, M. Ohtsuki, and Y. Hoshino FTY720, a Novel Immunosuppressant, Induces Sequestration of Circulating Mature Lymphocytes by Acceleration of Lymphocyte Homing in Rats. I. FTY720 Selectively Decreases the Number of Circulating Mature Lymphocytes by Acceleration of Lymphocyte Homing J. Immunol., May 15, 1998; 160(10): 5037 - 5044. [Abstract] [Full Text] [PDF]

				L. M. McEvoy, M. A. Jutila, P. S. Tsao, J. P. Cooke, and E. C. Butcher Anti-CD43 Inhibits Monocyte-Endothelial Adhesion in Inflammation and Atherogenesis Blood, November 1, 1997; 90(9): 3587 - 3594. [Abstract] [Full Text] [PDF]

				L. M. McEvoy, H. Sun, J. G. Frelinger, and E. C. Butcher Anti-CD43 Inhibition of T Cell Homing J. Exp. Med., April 21, 1997; 185(8): 1493 - 1498. [Abstract] [Full Text] [PDF]

				E Barbe, I Huitinga, E. Dopp, J Bauer, and C. Dijkstra A novel bone marrow frozen section assay for studying hematopoietic interactions in situ: the role of stromal bone marrow macrophages in erythroblast binding J. Cell Sci., January 12, 1996; 109(12): 2937 - 2945. [Abstract] [PDF]

				S. Rosen, M. Singer, T. Yednock, and L. Stoolman Involvement of sialic acid on endothelial cells in organ-specific lymphocyte recirculation Science, May 24, 1985; 228(4702): 1005 - 1007. [Abstract] [PDF]