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Somatic Cell Hybrids Between Mouse Peritoneal Macrophages and SV40-Transformed Human Cells

III. Identification of Surface Antigens Coded for by Human Chromosomes 7 and 17¹

Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, Pennsylvania 19104

Abstract

Somatic cell hybrids between SV40-transformed human cell lines and mouse peritoneal macrophages (MPM) containing either human chromosome 7 or 17 carrying the SV40 genome were injected into mice syngeneic to the mouse parental cells. Since either chromosome 7 or 17 was the only human chromosome present in the hybrids used as immunogens, the humoral immune response to gene products coded for by either chromosome was assayed. Using a sensitive radioimmunoassay, we were able to identify noncross-reactive cell-surface antigen(s) specifically coded for by either human chromosome 7 or 17, and present in normal, tumorderived and virus-transformed human cells. However, no reactivity against SV40 tumor-specific surface antigen (TSSA) could be detected in the antisera.

Footnotes

¹ This work was supported in part by research grants from the United States Public Health Service (CA 10815, CA 16685, GM 20700, and RR 05540), the National Foundation-March of Dimes (Basil O'Connor Starter Grant to CMC), by funds from the Commonwealth of Pennsylvania and by Grant VC 220 from the American Cancer Society.

² C.M.C. is a recipient of a Research Career Development Award (IK04 CA 00143) from the National Cancer Institute. Please send reprint requests to Dr. Croce.