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The Journal of Immunology, 1977, 118: 1335-1337.
Copyright © 1977 by The American Association of Immunologists, Inc.

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Hapten-Specific Leukocyte Migration Inhibition

I. Inhibition of Cells from Animals Immunized with DNP-KLH by {varepsilon}-DNP-L-Lysine Ficoll

Philip R. B. McMaster1, James D. Owens and Richard Asofsky

From the Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Abstract

Guinea pigs were immunized with dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH) or with hemocyanin in complete Freund's adjuvant. The migration of oil-induced peritoneal exudate cells was measured in the presence and absence of {varepsilon}-dinitrophenyl-lysine-tyrosinyl Ficoll (DNPL-F). When the cells came from animals immunized with DNP-KLH their migration was inhibited by DNPL-F. Control cells from animals immunized with KLH or not immunized migrated normally in the presence of DNPL-F.

Footnotes

1 Present address: Department of Clinical Pathology, National Institutes of Health, Bethesda, Maryland 20014.







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