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Recall of Specific Suppression: Co-Dominance of Suppression after Primary or Secondary Antigen Stimulation¹

From the Department of Bacteriology, University of California, Los Angeles, California 90024

Abstract

Mouse spleen cells primed in vivo with -galactosidase (GZ), and cultured in vitro with trinitrophenyl -galactosidase (TNP-GZ), make a very poor anti-TNP response compared to cultures of normal spleen cells. The lack of response is caused by active suppression as indicated by cell mixture experiments. At 2-months following priming, while the helper effect predominates, suppression can be recalled by rechallenge of the mice with GZ. Both primary suppression (after a single injection) and secondary suppression (after two injections) reduce the response of normal or carrier-primed helper cells at equivalent cell ratios in mixture experiments. Both kinds of suppression are carrier specific, selectively suppress high avidity antibody production, and require cell division to suppress the response of normal cells. The only difference between primary and secondary suppression is that primary suppression is obliterated in cultures which are not challenged with antigen until 24 hr after cultures are established. Secondary suppression is still observed when cultures receive TNP-GZ at 24 hr. Suppression appears to be an early component of both primary and secondary responses. The regulatory function of such suppression may be the delay of antibody secretion.

Footnotes

¹ This work was supported by Grant AI 11183 from the National Institutes of Health.

² Recipient of a Damon Runyon Postdoctoral Fellowship. Current address: Department of Pathology, Yale Medical School, New Haven, Connecticut 06510.