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A Microtiter Solid Phase Radioimmunoassay for Detection of the Human Reovirus-Like Agent in Stools

From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases and the Children's Hospital National Medical Center of the District of Columbia

Abstract

The development of a microtiter solid-phase radioimmunoassay for detection of the human reovirus-like (RVL) agent is described. The test is simple to perform and uses small amounts of reagents; a large number of specimens can rapidly be tested in a single test. Both filtered and unfiltered stool suspensions can be employed. The test was as sensitive as immune electron microscopy, and with an appropriate blocking test, was specific for the human RVL agent.

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				R. Yolken, B Barbour, R. Wyatt, A. Kalica, A. Kapikian, and R. Chanock Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species Science, July 21, 1978; 201(4352): 259 - 262. [Abstract] [PDF]