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A Comparative Study of Detection Methods for Aleutian Disease Viral Antibody¹

From the Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99163, Department of Pathology, University of California School of Medicine, Los Angeles, California 90024, Animal Pathology Division, Health of Animals Branch, Agriculture Canada, Animal Disease Research Institute (Western), Lethbridge, Alberta, Canada T1J 3z4

Abstract

Four methods of detecting and quantitating mink antibody against Aleutian disease (AD) virus were compared. Counterelectrophoresis, modified counterelectrophoresis, immunofluorescence, and complement fixation were performed blindly on 274 serum samples. All four methods were reliably specific for AD antibody. Immunofluorescence was less reproducible than the other systems. Immunofluorescence and complement fixation were 4- to 8-fold more sensitive than regular or modified counterelectrophoresis, but were limited by background staining and anti-complementary activity, respectively, when used to detect small amounts of antibody in undiluted sera.

Footnotes

¹ This work was supported in part by Public Health Service Research Grants AI-06477, AI-09476, and the Agricultural Research Service, United States Department of Agriculture.