HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Simon, P. L. Articles by Kind, P. D. Search for Related Content PubMed Articles by Simon, P. L. Articles by Kind, P. D.

The Xenogeneic Effect

III. Induction of Cell-Mediated Cytotoxicity by Alloantigen-Stimulated Thymocytes in the Presence of Xenogeneic Reconstitution Factor

From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, and the Department of Microbiology, The George Washington University Medical Center, Washington, District of Columbia

Abstract

A supernatant from human mixed leukocyte cultures (MLC), Xenogeneic Reconstitution Factor (XRF), was added to one-way murine MLC. This supernatant greatly enhances the generation of cytotoxic cells from C57BL/6 or DBA/2 responder thymocytes, as assayed by a standard ⁵¹Cr-release assay. This enhancement is shown 1) to be dependent upon the presence of BDF₁ semi-allogeneic stimulator cells and 2) to result in the generation of cytotoxic cells that are specific for the H-2 type of the stimulating alloantigen. In some experiments, alloantigen-stimulated murine spleen cells, when cultured with XRF showed increased induction of cell-mediated cytotoxicity. These experiments show that XRF contains a cytotoxic-cell activating factor(s) that is functionally similar to those found in murine-derived supernatants, and that this factor can participate in the generation of cytotoxic cells from precursors present in the murine thymus.

Footnotes

¹ This paper represents partial fulfillment of the requirements for the Ph.D. degree in the Department of Microbiology, George Washington University, Washington, District of Columbia.

² Address reprint request to: Philip Simon, Bldg. 30, Room 326, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014.