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Characterization of the Blastogenic and Cytotoxic Responses of Normal Mice to Ecotropic C-Type Viral gp71¹

Basic Research Program, NCI Frederick Cancer Research Center, Frederick, Maryland 21701

Abstract

Spleen cells from normal (B6C3)F₁ mice demonstrated natural cytotoxic reactivity mediated by a "null" cell population which, in part, had immunologic specificity for the major envelope glycoprotein (gp71) of the endogenous ecotropic murine leukemia virus. In contrast, the cytotoxic reactivity reflected in spleen cells of NIH Swiss nude mice apparently had no immunologic specificity. A significant level of blastogenic response could be generated in vitro by using normal (B6C3)F₁ and AKR spleen cells in the presence of gp71. This reactivity was highly type specific. Moreover, using normal spleen cells from (B6C3)F₁, both secondary blastogenic responses and cytotoxic T cell responses could be induced in vitro with purified soluble viral gp71. These findings extend further our previous studies on the existence of a natural immune response in normal mice to their endogenous MuLV by providing in vitro evidence for the expression of cell-mediated response in addition to the humoral response and that at least two effector cell types are operable in the cell-mediated phase.

Footnotes

¹ This work was supported by the National Cancer Institute under Contract No. NO1-CO-25423 with Litton Bionetics, Inc.

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