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Resistance to Murine Leukemia in Mice Receiving Simultaneous Injections of Syngeneic Hybrid and Parental Neoplastic Cells¹

From the La Rabida-University of Chicago Institute and the Department of Microbiology, University of Chicago, Chicago, Illinois 60649

Abstract

(A/J x C3H/HeJ)F₁ mice (H-2^a/k) exhibit no apparent resistance to the malignant proliferation of ASL-1 leukemia cells (H-2^a), a spontaneously occurring neoplasm of A/J mouse origin. The period of survival after injections of ASL-1 cells is related to the number of cells introduced into the susceptible host. (A/J x C3H/HeJ)F₁ mice receiving viable ASL-1 cells along with ASL-1 x LM(TK)^- somatic hybrid cells (H-2^a/k), syngeneic with the recipients, survived for statistically significant (p < 0.001), longer periods than did mice receiving ASL-1 cells alone. Mice receiving ASL-1 and ASL-1 x LM(TK)^- hybrid cells together survived for approximately twice as long as animals receiving ASL-1 cells alone. Mice receiving ASL-1 cells followed by hybrid cells survived for longer periods as well, but the therapeutic effects were less successful. ASL-1 x LM(TK)^- hybrid cells are rejected after forming localized tumors by the immunocompetent F₁ mice. In some instances, F₁ animals previously rejecting hybrid cells and challenged subsequently with ASL-1 cells along with a second injection of hybrid cells survived indefinitely. The passive transfer of partial immunity to the leukemic cells could be achieved by injections of spleen cells from F₁ mice that had rejected hybrid cells previously. Similar results were obtained for (A/J x C3H/HeJ)F₁ mice receiving RADA-1 cells (H-2^a), a radiation-induced leukemic line of A/J origin, along with RADA-1 x LM(TK)^- hybrid cells (H-2^a/k). The partial immunity induced by these lines of hybrid cells was cross-reactive: mice injected with ASL-1 cells and RADA-1 x LM(TK)^- hybrid cells survived for prolonged periods, and vice versa.

Footnotes

¹ This work was supported by the Leukemia Research Foundation, Inc., and the University of Chicago Cancer Research Center (United States Public Health Service Grant 5 PO1 CA 14599-03).

² Send reprint requests to: Dr. Edward P. Cohen, La Rabida-University of Chicago Institute, East 65th Street at Lake Michigan, Chicago, Illinois 60649.