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The Journal of Immunology, 1977, 118: 840-845.
Copyright © 1977 by The American Association of Immunologists, Inc.
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Regulation of Antibody Response in Different Immunoglobulin Classes

II. Induction of in Vitro IgE Antibody Response in Murine Spleen Cells and Demonstration of a Possible Involvement of Distinct T-Helper Cells in IgE and IgG Antibody Responses1

Masao Kimoto, Tadamitsu Kishimoto, Sanai Noguchi, Takeshi Watanabe and Yuichi Yamamura

From the Third Department of Internal Medicine, Osaka University Medical School, Fukushima-ku, Osaka, Japan

Abstract

In vitro induction of anti-DNP IgE as well as IgG1, IgG2a antibody responses was shown in murine spleen cell culture. Spleen cells primed three times with 1 µg of DNP-OA or DNP-Asc produced significant amounts of anti-DNP IgE as well as IgG antibodies by the in vitro stimulation with DNP-OA or DNP-Asc, respectively. Collaboration between DNP-primed B cells and carrier-primed T cells was required for the induction of both IgE and IgG antibodies with DNP-coupled T-dependent antigen. Carrier-specific T cells induced with a low dose of Asc (0.01 µg) showed helper function only on IgE antibody response, whereas T cells primed with a higher dose of Asc (10 µg) cooperated only with IgG-B cells. T cells primed with Asc in CFA showed helper function mainly on IgG antibody response but not on IgE antibody response. The result indicated the presence of a distinct population of T helper cells for IgE and IgG antibody responses. T-independent antigen (DNP-Ficoll) induced both anti-DNP IgE and IgG antibody responses in DNP-primed spleen cell population without the requirement of the collaboration of helper T cells.

Footnotes

1 This work was supported by a Grant-in-Aid for immunological research from the Ministry of Education, Science and Culture, Japan.






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