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Solubilization and Partial Characterization of Cell Membrane Fc Receptors¹

From the National Jewish Hospital and Research Center, Denver, Colorado 80206

Abstract

We assayed detergent cell lysates and culture supernatant fluid of mouse cells for the presence of soluble FcR by means of a radioligand bioassay. Strict correlation was found between the amount of FcR present in soluble form and that found on intact cells. The soluble FcR of all cells tested, except mouse macrophages, behaved as lipoprotein by buoyant density centrifugation analysis and enzyme treatment. In the absence of detergent, soluble FcR sedimented as a 20S macromolecule; in detergent the material was 7 to 9S. It was readily bound by insolubilized IgG but not by insolubilized BSA or F(ab')₂ fragments of IgG.

Footnotes

¹ This work was supported in part by United States Public Health Service Grants AI 09758 and CA 15895.

² Special Fellow of the Leukemia Society of America.