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A Disaccharide Hapten from Streptococcal Group C Carbohydrate that Cross-Reacts with the Forssman Glycolipid¹

From The Rockefeller University, 1230 York Avenue, New York, New York 10021

Abstract

A disaccharide hapten was isolated in approximately 20% yield from an acid hydrolysate of the streptococcal Group C carbohydrate. This disaccharide was assigned the structure 3-0--N-acetylgalactosaminosyl-N-acetylgalactosamine on the basis of chemical and immunochemical data. The ability of the hapten to inhibit completely the binding between Group C carbohydrate and most Group C antibodies indicated that this disaccharide is the immunodominant feature of the Group C carbohydrate. Fractionation of antisera on an -N-acetylgalactosamine immunoadsorbent column yielded several populations of anti-Group C antibodies. The reaction between labeled Group C carbohydrate and antibodies which strongly bound the immunoadsorbent column was 50% inhibitable by the disaccharide hapten when the hapten was added in approximately 15-fold excess (w/w) of the labeled antigen. On the other hand, the binding of those antibodies which did not bind to the immunoadsorbent column was poorly inhibited under these conditions. The Forssman glycosphingolipid, which has a common terminal digalactosamine unit, was shown to likewise inhibit Group C carbohydrate binding reactions.

Footnotes

¹ This work was supported by Grant PCM75-2131) from the National Science Foundation, Washington, D. C. and by Grant AI08429 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health.

² Recipient of a Postdoctoral Research Fellowship from the National Institutes of Health, National Institute of Allergy and Infectious Diseases AI05181

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