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Generation of Human Plaque-Forming Cells in Culture: Tissue Distribution, Antigenic and Cellular Requirements¹

From the Research Institute, Department of Immunology, The Hospital For Sick Children, Toronto, Ontario, Canada, M5G 1X8

Abstract

A system for the induction of specific, hemolytic plaque-forming cells from normal human lymphocytes in vitro (HcPFC) has been established and cells from various normal lymphoid tissues have been investigated. Normal values for anti-SRBC HcPFC responses in cultures of 10⁷ Ficoll-Hypaque separated lymphocytes range from 2000 (bone marrow) to 7000 (spleen) and 15,000 (tonsillar and peripheral blood lymphocytes). HcPFC responses to ovalbumin were lower by a factor of 2 to 4. Anti-SRBC as well as anti-ovalbumin responses required the cooperation of T lymphocytes and IgM-bearing B lymphocytes and the magnitude of the response was antigen dose dependent. Addition of adherent cells as well as of 2-mercaptoethanol enhanced the response. On the basis of the data obtained in experiments examining the role of B and T lymphocytes, a tentative model of cellular interaction has been postulated, suggesting a major role for antigen concentration in the modulation of the response via reactive T lymphocytes.

Footnotes

¹ This work was supported by a Grant MA 4875 from the Medical Research Council of Canada.

² Dr. Dosch is a fellow of the Deutsche Forschungsgemeinschaft.

³ Dr. Gelfand is recipient of a Queen Elizabeth II Scientist Award. Please send correspondence to: Dr. E. W. Gelfand, Department of Immunology, The Hospital For Sick Children, 555 University Avenue, Toronto, Ontario, Canada, M5G 1X8.

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