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Reaginic Antibody Formation in the Mouse

IX. Enhancement of Suppressor and Helper Cell Activities of Primed Spleen Cells¹

From the Department of Medicine, The Johns Hopkins University, School of Medicine at the Good Samaritan Hospital, Baltimore Md. 21239

Abstract

Attempts were made to increase the activity of suppressor cells in vitro. Antigen-specific suppressor cells were induced by i.v. injections of urea-denatured ovalbumin (UD-OA) into OA-primed mice. Nonadherent splenic lymphocytes from the UD-OA-treated mice were incubated at 37°C for 24 hr with either OA or OA-bearing macrophages and lymphocytes harvested from the culture were examined for the ability to suppress primary anti-hapten antibody response of nonirradiated mice to DNP-OA. The results showed that the suppressive activity of the lymphocytes increased after culture of the cells with OA or OA-bearing macrophages. Similar results were obtained when nylon column-purified T cell-rich fraction of the lymphocytes were similarly cultured. The suppressive activity was associated with -bearing lymphocytes and was specific for OA. Suppressor cells were not induced by the culture of OA-primed lymphocytes with OA. The helper function of splenic lymphocytes from both UD-OA-treated mice and OA-primed mice was enhanced by the culture of the cells with OA-bearing macrophages but not by culture with OA in the absence of macrophages.

Footnotes

¹ This work was supported by Research Grant AI-11202 from the United States Public Health Service and a grant from the John A. Hartford Foundation. This is publication No. 250 from the O'Neil Laboratories of the Good Samaritan Hospital.