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Cell-Mediated Immune Response in Vitro

II. The Mechanism(s) Involved in the Suppression of the Development of Cytotoxic Lymphocytes

Laboratory of Cellular and Comparative Physiology, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Public Health Service, United States Department of Health, Education and Welfare, Bethesda and Baltimore City Hospitals, Baltimore, Maryland 21224

Abstract

Spleen cells harvested from mixed leukocyte cultures (MLC) on day 2 or 3 suppress the development of CL from a fresh MLC across a cell-impermeable membrane, but day 4 MLC cells which have the maximum level of CL showed only a limited effect. Inhibition was observed only when suppressor cells were restimulated with the same H-2 type cells used during induction. However, the suppressive effect was not strain specific; that is, CBA-induced C57BL/6 spleen cells effectively inhibited the development of CL from DBA/2-induced C57BL/6 cells. In addition, DBA/2-induced C57BL/6 spleen cells effectively inhibited the development of CL from CBA cells. B10 spleen cells stimulated by B10.D2 cells gave rise to a suppressor cell population, indicating that H-2 differences alone can induce the response. The suppressive effect seemed to be exerted on an early phase of the response since no detectable inhibition was observed when suppressor cells were added 48 hr after culture initiation. The suppressive effect is not exerted on the accessory cell function but seems to inhibit DNA synthesis of the reacting cells in the MLC.

Footnotes

¹ The Third Department of Internal Medicine, Osaka University Hospital, Dojima, Fukushima-ku Osaka, Japan.

² Send correspondence to: Dr. A. A. Nordin, Laboratory of Cellular and Comparative Physiology, Geronotology Research Center, Baltimore City Hospitals, Baltimore, Maryland 21224.