HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Arbogast, B. W. Articles by Kefalides, N. A. Search for Related Content PubMed Articles by Arbogast, B. W. Articles by Kefalides, N. A.

The Role of Hydroxylation of Proline in the Antigenicity of Basement Membrane Collagen¹

From the Philadelphia General Hospital, General Clinical Research Center, and the Departments of Medicine and Biochemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Abstract

Rabbit antibodies to bovine basement membrane collagen were used to compare the antigenic determinants of rat parietal yolk sac basement membrane [¹⁴C]procollagen with [¹⁴C]protocollagen. Basement membrane [¹⁴C]protocollagen was found to be less antigenic than basement membrane [¹⁴C]procollagen. Hydroxylation of basement membrane [¹⁴C]protocollagen, either intracellularly or in vitro with protocollagen prolyl hydroxylase, resulted in restoration of antigenicity. The difference in antigenicity observed between basement membrane [¹⁴C]procollagen and basement membrane [¹⁴C]protocollagen appeared to depend primarily upon the presence of hydroxyproline in the collagen molecule. Glucosylgalactosylhydroxylysine was found to be unimportant for antigenicity.

Footnotes

¹ This work was supported by National Institutes of Health Grants AM-14526. and HL-15061.

² To whom correspondence should be addressed at Philadelphia Genreal Hospital, 700 Civic Center Boulevard, Philadelphia, Pennsylvania 19104.