HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Uzgiris, E. E. Articles by Kaplan, J. H. Search for Related Content PubMed Articles by Uzgiris, E. E. Articles by Kaplan, J. H.

Tuberculin-Sensitized Lymphocytes Detected by Altered Electrophoretic Mobility Distributions after Incubation with the Antigen PPD¹

From the General Electric Corporate Research and Development, Schenectady, New York 12301

Abstract

Lymphocytes from donors who had had tuberculosis (a disease known to provoke a cell-mediated immunity) were incubated with the tuberculin antigen, purified protein derivative (PPD), and their distribution of electrophoretic mobility was determined by laser Doppler spectroscopy. In 75% of the cases, the distribution showed a new, high mobility cell subpopulation that was not present before exposure to the PPD. Control lymphocytes from individuals with negative skin tests or no record of tuberculosis showed no mobility changes after incubation with PPD. These observations indicate that the new mobility subpopulation arose from a specific interaction between the antigen and sensitized cells of the donors.

Footnotes

¹ This work was supported in part by the Molecular Control Program of the National Cancer Institute under Contract NO1-CP-3-3231.