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Trypsin-Induced Increase in Intracellular Cyclic AMP of Lymphocytes¹

From the Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel

Abstract

Trypsin increases intracellular levels of cyclic AMP (cAMP) in lymphocytes. The trypsin-induced increase in cAMP is blocked by specific trypsin inhibitors and by high concentrations of different proteins. Several proteolytic enzymes from various sources, including other pancreatic proteases, do not cause an increase in cAMP under the same experimental conditions. Immobilized trypsin induces the same increase in cAMP as does free trypsin. The trypsin-induced rise in cAMP is not due to inhibition of cAMP phosphodiesterase, but consistent activation of adenylate cyclase by trypsin could not be demonstrated. The extent of the trypsin-induced increase in intracellular cAMP correlates with the type of the lymphocyte and with the state of maturity attained by the cells. Transformed lymphocytes and nonlymphoid cells do not react at all.

Footnotes

¹ This study was supported by a grant from the Israel Commission for Basic Research.