HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Fairchild, S. S. Articles by Malley, A. Search for Related Content PubMed Articles by Fairchild, S. S. Articles by Malley, A.

Mouse B and T Lymphocyte Responses to Purified Timothy Pollen Antigens in Vitro¹

From the Oregon Regional Primate Research Center, Beaverton, Oregon 97005

Abstract

Purified populations of splenic B and T lymphocytes from LAF₁ mice immunized with a crude extract of timothy pollen (WST) responded specifically to pollen antigens in an in vitro lymphocyte transformation system. The peak lymphocyte transformation response occurred 5 days after a secondary immunization and was the result of T-B cell cooperation in vitro. With two purified pollen antigens as in vitro stimulants we were able to define at least two antigen-specific populations of B cells and one population of T cells. These results were confirmed by inhibition studies with a monovalent hapten from WST, Antigen D.

Footnotes

¹ This work was supported by Public Health Service Grant AI09232-04 and Oregon Regional Primate Research Center Grant RR-00163-16 and is Publication No. 879 of the Oregon Regional Primate Research Center.

² This work was in partial fulfillment of Ph.D. requirements at the University of Oregon Medical Center, Portland, Oregon; current address: Division of Clinical Immunology, University of Colorado Medical Center, Denver, Colorado 80220.

³ Reprint requests should be sent to Dr. A. Malley, Oregon Regional Primate Research Center, Beaverton, Oregon 97005.