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Idiotypes of Inulin-Binding Antibodies and Myeloma Proteins Controlled by Genes Linked to the Allotype Locus of the Mouse

From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Abstract

The serum from non-immunized mice of strains BALB/c, C58, A/He, and RIII contained hemagglutinins for stearoyl inulin-coated SRBC. Immunization with bacterial levan slightly elevated these titers. These same sera also carried cross-specific idiotypic determinants (IdX) that are associated only with inulin-binding myeloma proteins (INBMP) of BALB/c mice. Three InuIdX specificities, A, B, and G, were identified. The InuIdX phenotypes of strains BALB/c, C58, and A/He were InuIdXA⁺B⁺G⁺; strain RIII was InuIdX A⁺B^-G⁺; strain C57BL/6, C57BL/10, DBA/2, AKR and NH were A^-B^-G^-. Strains CBA, C3H, PL, and C57L could not be typed because of low and inconsistent levels of InuIdX and anti-inulin hemagglutinins.

The InuIdXA⁺B⁺G⁺ phenotype was used as a genetic marker in immunoglobulin congenic strains CB-20, BAB-14, and BC-8 and in Bailey RI strains which are derived from crosses of BALB/c (InuIdXA⁺B⁺G⁺) and C57BL/ka or C57BL/6, respectively (InuldXA^-B^-G^-). Linkage of the IdXA⁺B⁺G⁺ to the BALB/c a¹ allotype locus was demonstrated. In addition, the InuldXA⁺B⁺G⁺ marker was used as a phenotype in an analysis of 168 first generation backcross progeny (C57BL x (C57BL x BALB/c) F₁). Linkage of the marker to the BALB/c allotype was found again. Two proven recombinant mice having the C57BL a² allotype and the InuIdxA⁺B⁺G⁺ markers were identified and progeny tested. Four other potential crossover types are still being progeny-tested.