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Isolation of a Human Lymphocyte Mitogen from Wheat Germ with N-Acetyl-D-Glucosamine Specificity¹

Departments of Biochemistry and Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201

Abstract

A lectin, isolated from wheat germ by affinity chromatography on chitin, was mitogenic for purified human peripheral blood lymphocytes. Peak incorporation of ³H-thymidine was observed after incubation of lymphocyte cultures with wheat germ mitogen for 7 to 10 days. When lymphocytes were separated into two fractions based on their ability to form rosettes with unsensitized sheep erythrocytes, the mitogen induced a negligible proliferative response in either fraction. Mixing experiments demonstrated a strong response in the T lymphocyte fraction which required the collaboration, but not proliferation, of cells present in the nonrosetting fraction. Stimulation was specifically abolished by addition of N-acetyl-D-glucosamine at initiation of culture.

Footnotes

¹ This work was supported by National Institutes of Health Grants CA 20812, AI 11923, AI 11013, AI 12887 and American Cancer Society Grant IM 68A.