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The Journal of Immunology, 1976, 117: 1953-1959.
Copyright © 1976 by The American Association of Immunologists, Inc.
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Antigen-Specific and Nonspecific Mediators of T Cell/B Cell Cooperation

IV. Development of a Model System Demonstrating Responsiveness of Two T Cell Functions to HGG in Vitro1

J. Terrell Hoffeld2, Philippa Marrack and John W. Kappler

Division of Immunology and the University of Rochester Cancer Center; and the Departments of Dental Research and Microbiology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642

Abstract

Previous work in this laboratory has defined two functionally distinct T cell subpopulations (viz., antigen-specific helper and antigen-nonspecific helper) and characterized some of the parameters of these subpopulations derived from the keyhole limpet hemocyanin (KLH)-primed mouse spleen. Characterization of further parameters such as relative susceptibility to tolerance induction and relative degree of specificity was not possible with the use of KLH as the antigen. In order to overcome this impediment, an experimental protocol was developed, as herewith described, to permit the study of these two T cell subpopulations in response to the antigen human {gamma}-globulin (HGG). This protocol qualitatively duplicates the parameters defined for the KLH system and provides an extremely useful model for the study of the response to serum proteins in the Mishell-Dutton culture system.

Footnotes

1 Various portions of the work have been supported by United States Public Health Service Research Grants AI-11558 and CA-11198 and American Cancer Society Research Grant IM-49. J.T.H. is supported by United States Public Health Service Training Grant 5-T01-DE00003-18.

2 Address reprint requests to: Dr. J. T. Hoffeld, Department of Dental Research, University of Rochester Medical Center, Rochester, New York 14642.






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