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Electrophoretic Separation of Radioisotopically Labeled Mouse Lymphocytes¹

Biophysics Laboratory, Department of Nutrition and Food Science, M.I.T., Cambridge, Massachusetts 02139 and the Departments of Nuclear Medicine and Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111

Abstract

A new preparative method is described for the separation and direct electrophoretic comparison of the distribution profile of radioisotopically labeled cells. The cell populations to be compared are labeled separately with different radioisotopes (e.g., ⁵¹Cr and ^99mTc) and mixed. The cell mixture is subjected to density gradient electrophoresis and the distribution of each radioisotope in the collected fractions is ascertained by differential gamma counting. In the case of uniform label uptake by the cells in each distribution, the radioactivity counts represent cell frequency. Nonuniform labeling allows useful comparisons only in terms of relative mobility distribution. Model experiments performed with ⁵¹Cr- and ^99mTc-labeled mouse thymocytes and spleen cells are in general agreement with mobility distribution results obtained previously by free flow electrophoresis.

Footnotes

¹ This work was supported by National Cancer Institute Contract NO1-CB-43928 and by a grant from the American Cancer Society (Massachusetts Division) and the National Institutes of Health (Ca16172-01).

² H.H.W. is a research fellow of the American Cancer Society.