The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1976, 117: 1938-1942.
Copyright © 1976 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Imir, T.
Right arrow Articles by Mäkelä, O.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Imir, T.
Right arrow Articles by Mäkelä, O.

Two Types of Antibody Dependent Cell-Mediated Cytotoxicity, Arming and Sensitization1

T. Imir2, Eero Saksela and O. Mäkelä

Department of Serology and Bacteriology and the Third Department of Pathology, University of Helsinki, 00290 Helsinki 29, Finland

Abstract

Haptenated chicken erythrocytes, anti-hapten antibodies and normal spleen cells were used for a study of parameters of antibody-dependent cell-mediated cytotoxicity (ADCC). Anti-hapten antibody was introduced to the reaction either bound to the target cells (sensitization) or to the effector cells (arming). Our data suggest that the same effector cells and the same antibodies were responsible for both types of reactions. More antibody was required for arming (ca. 1 ng/ml) than for sensitization (ca. 10 pg/ml).

Antibodies of several mammalian species could cooperate in ADCC with human, rat, or guinea pig spleen effector cells, but fowl antibodies were inefficient in the arming of mammalian effector cells.

Rat and human spleen cells were powerful effector cells whereas guinea pig, rabbit, and mouse spleens yielded weakly cytotoxic suspensions. Rabbit antibodies, on the other hand, were efficient, but rat antibodies were less efficient in ADCC. Mouse and guinea pig antibodies appear to take an intermediate position.

Our data suggest that ADCC effector cells of the spleen contain phagocytic and nonphagocytic cells, and the non-phagocytic effector cells still constitute a heterogeneous population.

Footnotes

1 This investigation was supported by a research contract with the Finnish Cancer Society.

2 Fellow of the Sigrid Jusélius Foundation. Permanent address: Hacettepe University, Medical Faculty. Institute of Microbiology, Ankara, Turkey.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1976 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1976 by The American Association of Immunologists, Inc. All rights reserved.