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The L1210 Leukemia Cell Bears a B Lymphocyte Specific, non-H-2 Linked Alloantigen^1,2,

Departments of Pharmacology and Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605; the Department of Clinical Medical Sciences, Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland 20014; and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Abstract

The L1210 murine lymphoblast cell line possessed a B lymphocyte-specific alloantigen which was detected with C57BL/Ks anti-L1210 serum. The antigen was found on splenic B lymphocytes but not on thymocytes, T lymphocytes, or erythrocytes. It was present on only 7% of bone marrow cells. The reactivity of C57BL/Ks anti-DBA/2-spleen serum was indistinguishable from that of the anti-L1210 serum, confirming that the antigen was a normal component of B lymphocytes of the DBA/2 mouse. The strain distribution pattern of the antigen detected by the C57BL/Ks anti-L1210 serum indicated that this alloantigen was not an allele of the H-2K, H-2D, Mls, or Ig loci. Genetic analysis indicated that the antigen was inherited as a single, Mendelian dominant trait. Segregation analysis of (B10.BR x DBA/2)F₁ x AKR/J offspring indicated that this B cell marker was was not linked to genes coding for H-2.31 or Ly 4.2. The C57BL/Ks anti-L1210 serum identified a new polymorphic genetic locus, the product of which was B lymphocyte specific.

Footnotes

¹ This work was supported by grants from The Anna Fuller Fund, The General Research Support Fund of the University of Massachusetts, and the American Cancer Society, Massachusetts Division and by the Bureau of Medicine and Surgery, Navy Department, Research Task No. MF51.524.013.1004. The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large.

² The animals used in this study were handled in accordance with the provisions of Public Law 89-44 as amended by Public Law 91-579, the "Animal Welfare Act of 1970" and the principles outlined in the "Guide for the Care and Use of Laboratory Animals," United States Department of Health, Education and Welfare Publication No. (MIH) 73-23.

³ Robert E. Humphreys was a recipient of a Cancer Research Scholar Award from the American Cancer Society, Massachusetts Division.