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Guinea Pig Lymphocyte-Derived Macrophage Aggregation Factor: Its Separation from Macrophage Migration Inhibitory Factor¹

From the Veterans Administration Hospital and Department of Medicine, University of Tennessee Center for the Health Sciences, Memphis, Tennessee 38104

Abstract

Lymphocytes from guinea pigs having delayed hypersensitivity to horse-radish peroxidase (HRPO) when cultured in vitro with HRPO produce a large m.w. factor (; 100,000 daltons) that causes peritoneal macrophages from nonimmune animals to aggregate. The macrophage aggregation factor (MAF) can be separated from macrophage migration inhibitory factor (MIF) by gel filtration of active lymphocyte supernatants on Sephadex G-150. MAF is heat stable (56°C for 30 min) but inactivated by trypsin.

These data suggest that aggregation of macrophages in vitro by lymphokine-rich culture supernatants is nót due to MIF but is caused by a separate large m.w. factor.

Footnotes

¹ This study was supported by Veterans Administration Research Projects 4826-01, 4826-02, and 7146-01, and by United States Public Health Service Grant AM-16506.

² Research Associate of the Veterans Administration.

³ Medical Investigator of the Veterans Administration.