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The Induction and Regulation of Guinea Pig B-Lymphocyte Proliferation in Vitro

From the Biologic Structure Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Conditions for the in vitro induction of proliferation have been examined in a population of guinea pig lymph node lymphocytes enriched for B lymphocytes. Known B cell mitogens such as bacterial lipopolysaccharide and tuberculin-purified protein derivative induced DNA synthetic responses in this B lymphocyte population as did a number of dinitrophenyl hapten-protein conjugates, all in the apparent absence of T cell participation. B cell proliferation occurred most efficiently in serum-free medium with both heterologous and homologous sera inhibiting maximum responses. In vivo immunization with hapten-protein conjugates failed to enhance subsequent in vitro B cell responsiveness to these materials beyond that owing to recruitment by contaminating antigen-primed T cells. Macrophage-associated antigen, which efficiently triggered T cell proliferation, was much less effective at initiating B cell DNA synthesis than was soluble antigen not bound to macrophages. Furthermore, although accessory cells were required for the development of T cell DNA synthetic responses, macrophages or factors produced by them inhibited spontaneous or induced B cell ³H-thymidine incorporation.