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Identification of Multiple Cytolytic Components Associated with the -LT Class of Lymphotoxins Released by Mitogen-Activated Human Lymphocytes in Vitro¹

From the Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California 92717

Abstract

Cytotoxic activity(lymphotoxin (LT) associated with the 50,000 dalton -LT class of human lymphotoxins has been resolved into multiple components by chromatography on DEAE-cellulose or electrophoresis on polyacrylamide gels. The two components, termed -LT₁ and -LT₂, are separable from the 90,000 m.w. -LT, since neither component is neutralized by incubation with heterologous rabbit anti--LT antisera. However, the two components differ in their overall charge and relative heat stability with the LT₁ component being more positively charged than the -LT₂ molecule and extremely heat labile.

Footnotes

¹ This research was supported by Grant AI-09460 from the Institute of Allergy and Infectious Diseases, National Institutes of Health and Grant No. IM-32, from the American Cancer Society.