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Characterization of Lymphocyte Inhibition by Supernatants of Crowded Lymphocytoblasts¹

From the Clinical and Experimental Immunology Department, Clinical Immunology Division, Naval Medical Research Institute, Bethesda, Maryland 20014

Abstract

Crowded lymphocytoblasts produce a factor (or factors) that inhibits the response of peripheral blood lymphocytes to mitogens. This factor is destroyed by pepsin in 1 hr but not by neuraminidase. No activity was lost when the factor was incubated with packed human red blood cells, human lymphocytes, mouse spleen cells, or tissue culture cells. An antiserum was made to the factor which was capable of partially inhibiting its activity. The factor is active at about 25 µg/ml of protein. Maximal inhibition occurs if the factor is present for the duration of the culture period. This factor may play a regulatory role in the immune response.

Footnotes

¹ This work was supported by the Naval Medical Research and Development Command, Work Unit No. MR041.02.01.0013A2JC. The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the United States Navy Department or the naval service at large. The animals used in this study were handled in accordance with the provisions of Public Law 89–54 as amended by Public Law 91–579, "Animal Welfare Act of 1970," and the principles outlined in the "Guide for the Care of Laboratory Animals," U. S. Department of Health, Education, and Welfare Publication No. (NIH) 73–23.

² Present address: United States Department of Agriculture, Animal and Plant Inspection Health Service, Washington, District of Columbia 20250.