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Partial Separation of Human Blood Leukocytes by Density Gradient Electrophoresis: Different Mobilities of Lymphocytes with IgG, Those with IgM and IgD, T Lymphocytes, and Monocytes¹

Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115 and the Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Abstract

Previous studies on the electrophoretic separability of human T and B peripheral lymphocytes have produced conflicting results. Vassar et al. (1) using the microscope chamber technique were unable to show any correlation between electrophoretic mobility and degree of enrichment in either T or B lymphocytes. In contrast to the above results, other laboratories (2–6) have shown charge differences between human T and B cells of such magnitude that suggest the possibility of complete separability of these two populations. However, the microscope chamber method does not provide actual physical separation of the electrophoresed cells. Recent attempts by Stein (7) and Häyry et al. (8) to separate physically T and B human lymphocytes by free flow electrophoresis resulted only in partial enrichment of these cells in certain fractions. In consideration of the above findings and in view of the importance of separating human lymphocyte subpopulations by physical methods, we have applied the recently developed technique of density gradient electrophoresis (9, 10), to examine the separability of these cells.

Footnotes

¹ This work was supported in part by National Institute of Health Contract N01-CB-43928 and Grant AI-10091, and by National Cancer Institute Grant 14723.