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In Vitro Responses of CBA/N Mice: Spleen Cells of Mice with an X-Linked Defect that Precludes Immune Responses to Several Thymus-Independent Antigens Can Respond to TNP-Lipopolysaccharide¹

Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014 and the Department of Clinical and Experimental Immunology, Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland 20014

Abstract

Spleen cells from CBA/N mice with an X-linked B cell defect were examined for their ability to form antibody in vitro after stimulation with the T-independent antigen TNP-LPS. In contradistinction to their failure to respond to some conventional T-independent antigens such as type III pneumococcal polysaccharide or DNP-AECM-Ficoll, spleen cells from (CBA/N x DBA/2)F₁ male mice were able to make a specific anti-TNP PFC response after culture with TNP-LPS. Their response differed from that of phenotypically normal (CBA/N x DBA/2)F₁ female litermate spleen cells in that more TNP-LPS was required to elicit the peak anti-TNP response and the anti-TNP antibody secreted by F₁ male cells was of lower avidity than that of F₁ female cells. The polyclonal antibody response to unsubstituted LPS did not differ substantially between normal and defective B cells. Thymus-derived cells were not required for the TNP-LPS response by either F₁ male or female cells. We conclude that CBA/N B cells can respond to certain T-independent antigens that are able either to induce a very strong activating signal upon ligand-surface receptor interaction and/or to stimulate immature B cells (with a characteristic high surface immunoglobulin profile) which fail to respond to antigens like DNP-AECM-Ficoll.

Footnotes

¹ This work was supported in part by the Naval Medical Research and Development Command, Work Unit No. MR041.02,01.0020. The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the U. S. Navy Department or the naval service at large.

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