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The Journal of Immunology, 1976, 117: 1356-1362.
Copyright © 1976 by The American Association of Immunologists, Inc.
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Release of Chemical Mediators from Partially Purified Human Lung Mast Cells1

Nigel A. M. Paterson2, Stephen I. Wasserman3, Jonathan W. Said and K. Frank Austen

Departments of Medicine and Pathology, Harvard Medical School; the Department of Medicine, Robert B. Brigham Hospital, and the Department of Pathology, Peter Bent Brigham Hospital, Boston, Massachusetts

Abstract

Human lung mast cells dispersed by enzymatic digestion of human lung fragments were concentrated to greater than 50% purity by sedimentation in isopycnic and velocity gradients. The dispersed lung mast cells had a characteristic ultrastructural appearance including granules with a scroll or reticular structure surrounded by perigranular membranes. Histamine and preformed eosinophilotactic activity sedimented with the mast cells on isopycnic gradients, and the mast cells and these mediators were separated from the bulk of the other lung cells after velocity gradient sedimentation. The histamine content of isolated lung mast cells was calculated to range from 1.0 to 5.5 pg/cell. The quantity of SRS-A generated with anti-IgE or specific antigen was relatively limited but confined to the mast cell-rich fractions and associated with the release of histamine and eosinophilotactic activity.

Footnotes

1 This work was supported by Grants AI-07722, AI-10356, and HL-17382 from the National Institutes of Health, Bethesda, Maryland.

2 Fellow of the Medical Research Council, Canada.

3 Research Fellow of The Arthritis Foundation.




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