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Characterization of Ia Antigens in Mouse Serum¹

Department of Molecular Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037, the Division of Immunochemistry, William Beaumont Hospital Research Institute, Royal Oak, Michigan 48072, the Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48202, and The Department of Microbiology, University of Texas, Southwestern Medical School, Dallas, Texas 75235

Abstract

Sera obtained from normal B10.BR mice were shown to inhibit selectively a specific anti-Ia alloantiserum. Partial purification of the Ia antigenic activity was accomplished by isolation of the high density lipoproteins from these sera by fractional precipitation with sodium phosphotungstate and MgCl₂. Both H-2.23 and Ia^k antigens present in this high density lipoprotein fraction were completely adsorbed by rabbit anti-rat ₂-microglobulin immunoadsorbents, whereas specific anti-H-2.23 immunoadsorbents removed only the H-2 activity. These data demonstrate that Ia antigens, like H-2 antigens in the sera of B10.BR mice are associated with high density lipoproteins and further suggest that both H-2 and Ia antigens are associated with a ₂-microglobulin-like molecule.

Footnotes

¹ This is Publication 1086 from Scripps Clinic and Research Foundation, La Jolla, California. This work was supported by United States Public Health Service Grants AI 11335, AI 12589, and AI 13154 from the National Institutes of Health, Childrens Leukemia Foundation of Michigan and The William Beaumont Hospital Research Institute.

² GNC is a recipient of National Institutes of Health Fellowship CA 00006. Please address correspondence: G. N. Callahan, Ph.D., Department of Molecular Immunology, Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, California 92037.

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