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Lysis of Tumor Cells by Antibody and Complement

VII. Complement-Dependent ⁸⁶Rb Release—a Nonlethal Event?

Laboratory of Immunobiology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Abstract

The guinea pig hepatoma (line-1) treated with anti-Forssman antibody (TA) and GPC sequentially released ⁸⁶Rb, ¹⁴C from ¹⁴C aminoisobutyric acid and failed to exclude trypan blue. Incubation of TA with fluid phase GPC for 1 min caused maximal ⁸⁶Rb release; however, if the GPC was removed at this time, the cells were not subsequently killed. Using a number of naturally occurring human sera deficient in a complement component we have shown ⁸⁶Rb release requires the binding of the complement components 1 through 8, but there was no absolute requirement for C9. Irreversible damage to the cell as measured by ¹⁴C AIB release or uptake of trypan blue required the complete sequence of complement components. These observations indicate that ⁸⁶Rb release is not a reliable indicator of cytotoxicity.