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The Journal of Immunology, 1976, 117: 1336-1339.
Copyright © 1976 by The American Association of Immunologists, Inc.
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Mitogen-Stimulated Glutaraldehyde-Fixed Spleen Cells: Ability to Stimulate in the Mixed Lymphocyte Reaction and Generate Effector Cells in Cell-Mediated Lympholysis1

James J. Lightbody and Yi-Chi M. Kong

Department of Biochemistry, and the Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201

Abstract

Mouse spleen cells treated with glutaraldehyde lose their stimulating ability in the MLR. If the spleen cells are first converted to a blastogenic state by lipopolysaccharide and subsequently fixed with glutaraldehyde, their stimulating capacity is maintained.

Footnotes

1 This work was supported by National Institutes of Health Grants AI 11923 and AI 11013, and by the Michigan Heart Association and Kidney Foundation of Michigan.






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