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B Lymphocyte Alloantigen Specificities Present on Cultured Lymphoblastoid Cell Lines¹

Department of Surgery, School of Medicine, University of California, Los Angeles, California 90024

Abstract

Thirty-three of 34 cultured human lymphoblastoid B cell lines have been shown to express four out of five polymorphic non-HLA specificities expressed by normal B lymphocytes. The specificities were detected by 32 alloantisera produced by absorption with pooled platelets to remove HLA activity and selected from over 400 pregnancy sera. One B group (B2) which was expressed by 23% of a panel of normal B lymphocytes was not found on any of the cultured lines tested. Four T cell lines tested and myeloid line (K562) did not react with any of the B cell alloantisera.

Footnotes

¹ This work was supported by United States Public Health Service Contract AI 12366-02 from the National Institutes of Health, Bethesda, Maryland.