HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Thursh, D. R. Articles by Chan, K. S. Search for Related Content PubMed Articles by Thursh, D. R. Articles by Chan, K. S.

Immunologic Cross-Reactions Between the Specifically Localizing Cells (SLC) Generated in Response to Sheep RBC and the Red Cells of Other Species

Department of Pathology, New York Medical College, Valhalla, New York 10595

Abstract

Some of the lymphoid cells selectively incorporating radioactive thymidine 3 days after primary immunization with SRBC which are capable of specifically localizing in the lymph nodes of adoptively immunized syngeneic recipients challenged with SRBC (SLC-SRBC)² are also capable of specifically localizing in lymph nodes challenged with the red blood cells of a variety of other mammalian species. The one nonmammalian RBC tested, CRBC, failed to cross-react with SRBC by this parameter even qualitatively, although it remains possible that a feeble cross-reaction might be demonstrated with large enough experimental groups. As expected, the magnitude of the observed cross-reactions seems to vary inversely with the phylogenetic distance between the species, with ORBC showing the strongest cross-reaction (41 to 49%), ARBC showing the weakest (8 to 13%), and BRBC and HRBC occupying intermediate positions (13 to 20%). Cross-reactions between secondary anti-SRBC antibodies and the red cells of the other species were weak and inconstant, suggesting that the observed cross-reactions of specific localization may involve T cells more than B cells. During the course of these studies, it was also possible to verify experimentally our impression that with existing methods of selectively labeling SLC it is possible to study the phenomenon of specific localization using only one population of labeled cells, providing certain essential controls are employed.

Footnotes

² Abbreviations used in this paper: ORBC, ox red blood cells; BRBC, burro red blood cells; HRBC, horse red blood cells; CRBC, chicken red blood cells; ARBC, human red blood cells (type A); BA, Brucella abortus; SLC*, radioactivity in dpm attributable to SLC.

¹ Holder of United States Public Health Service Award AI-70789 and a Career Scientist Award from the Irma T. Hirschl Trust.