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Effects of C-Reactive Protein on Platelet Function

II. Inhibition by CRP of Platelet Reactivities Stimulated by Poly-L-Lysine, ADP, Epinephrine, and Collagen^1,2,

Departments of Immunology, Rush Medical College, and Microbiology, University of Illinois at the Medical Center, Chicago, Illinois 60612

Abstract

It was recently demonstrated that C-reactive protein (CRP)⁴ inhibits the response of human platelets to heat-aggregated human -globulin and thrombin and that this inhibition is characterized by a dose-dependent reduction in aggregation, activation of platelet factor 3 (PF3), and release of -glucuronidase. In the present experiments, CRP was found also to inhibit the ability of washed human platelets to aggregate in response to poly-l-lysine (PLL); in these experiments, the magnitude of the inhibitory effect was dependent upon the m.w. of PLL used as the stimulating agent, and was more effective with low (15,000 daltons) than with high (400,000 daltons) m.w. polymers. CRP similarly inhibited ADP- and epinephrine-stimulated platelet aggregation in platelet-rich plasma (PRP), and this was characterized by relatively minimal suppression of the primary wave with marked suppression of the secondary ADP-mediated wave of aggregation. CRP also inhibited the platelet aggregation induced by collagen in PRP, although it had no effect upon the adherence of platelets to collagen. Finally, CRP inhibited the activation of PF3 and the release of serotonin during stimulation of platelets with ADP, and this inhibition was temporally related to the onset of the secondary wave of aggregation. These experiments extend the platelet reactivities inhibited by CRP, show that CRP expresses its inhibitory capacity in platelet-rich plasma as well as upon isolated platelets, raise the possibility that CRP exercises its effects by inhibiting or interfering with the release and/or utilization of endogenous platelet ADP, and support the concept that CRP plays an important role in the control of platelet responsiveness to a variety of stimuli during acute inflammatory reactions.

Footnotes

¹ This work was supported by grants from the National Institutes of Health (AI 12870-01), Chicago Heart Association, Leukemia Research Foundation, Inc., and Hunter Trust.

² Presented in part to the American Association of Immunologists, April 12, 1976.

⁴ Abbreviations used in this paper: ADP, adenosine diphosphate; AHGG, aggregated human -globulin (Cohn fraction II); CRP, C-reactive protein; 5HT, 5-hydroxytryptamine or serotonin; LDH, lactic dehydrogenase; PF3, platelet factor 3; PLL, poly-L-lysine; PPP, platelet-poor plasma; PRP, platelet-rich plasma.

³ Holder of the Thomas J. Coogan, Sr. Chair of Immunology established by Marjorie Lindheimer Everett.

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