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The Journal of Immunology, 1976, 117: 847-851.
Copyright © 1976 by The American Association of Immunologists, Inc.
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Synthetic Glycolipid Adjuvants1

Ulrich H. Behling, Barbara Campbell, Chung-Ming Chang, Christopher Rumpf and Alois Nowotny

Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

Abstract

In the course of the organic synthesis of model compounds similar in some features to the lipid moiety of endotoxic lipopolysaccharide (LPS), N-acylated-D-glucosamine derivatives were prepared. One of these, N-palmitoyl-D-glucosamine, has been previously found to be mitogenic for athymic nude mouse B cells. This and other N-acylated homologs were tested for adjuvant activity in the immune response to human {gamma}-globulin (HGG) and sheep red blood cells (SRBC) in mice. Comparable or superior enhancement of the immune response was obtained for these glycolipids when compared to LPS in assays measuring anti-SRBC or HGG hemagglutinin titers. In the determination of hemolytic plaque formation, considerable adjuvant effect was shown by the lauroyl derivative, and less but still significant enhancement was achieved by the N-palmitoyl-d-glucosamine. In the rosette formation assay, in addition to the above two glycolipids, N-oleyl-D-glucosamine showed good adjuvant effect. In the latter two assays, the LPS was a superior adjuvant as compared to the synthetic glycolipids.

The radiation protective effect of some of the better synthetic adjuvants was also investigated in mice. It was found that although LPS was more effective in this assay, the N-myristoyl-D-glucosamine and N-decanoyl-D-glucosamine compounds gave a definite protection, since up to 40% of the lethally irradiated (700 R) mice survived.

Footnotes

1 This work was supported by United States Public Health Service Grant 5 R01 CA 16934.






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