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Immune Response in the Hamster

VIII. Ig Class Differences in Susceptibility to Tolerance Induction

United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana 59840

Abstract

A hemolytic plaque assay was used to quantitate the antibody response of the Syrian hamster after immunization with hen egg albumin (HEA). Whereas HEA in complete Freund's adjuvant (HEA-CF) induced a prolonged heterogeneous (IgM, IgG₁ and IgG₂) antibody response, the response to soluble HEA in saline (HEA-S) differed in that: 1) both the primary and secondary responses were restricted to the IgG₁ class; 2) the IgG₁ primary response was cyclical with PFC peaks on days 9 and 16; 3) although an anamnestic secondary response was demonstrated, no further augmentation was noted after tertiary and quaternary boosters; 4) the booster response was transient reaching a peak after 48 hr and declining to low levels within 7 days. Adoptive transfer of lymph node cells to irradiated recipients followed by challenge with HEA-CF revealed: 1) that HEA-S-treated donor cells were primed for an IgG₁ response because anamnesis was seen 7 days after challenge, yet on day 21, IgG₁ PFC were 20-fold less than that of controls; 2) recipients of HEA-S treated cells showed profound suppression of both IgM and IgG₂ PFC on days 14 and 21. These studies indicate that soluble antigen induced in hamsters a state of complete tolerance of IgM and IgG₂ classes whereas the anamnestic response of the IgG₁ class remained intact.

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