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In Vitro Induction of F₁ Hybrid Anti-Parent Cell-Mediated Cytotoxicity¹

Immunology Branch, National Cancer Institute, Bethesda, Maryland 20014 and the Department of Pathology, School of Medicine, State University of New York at Buffalo, Buffalo, New York 14214

Abstract

Spleen cells from normal (C57BL/6 x DBA/2)F₁ mice were sensitized in vitro for 5 days with irradiated C57BL/6 or DBA/2 parental stimulating cells. Effector cells were generated which specifically lysed ⁵¹Cr-labeled targets (leukemia or mitogen-stimulated lymphoid cells) H-2-matched with the parental genotype used for sensitization. The response of F₁ spleen cells to the C57BL/6 parent was stronger and more reproducible than that to the DBA/2 parent. The kinetics of generation of effector cells were similar for the F₁ anti-parent and an F₁ anti-allogeneic response. However, the magnitude of the F₁ anti-C57BL/6 cytotoxic response was considerably lower than the F₁ response to allogeneic cells. The ratio of responder to stimulator cells in the cultures was more critical for the former than for the latter response. Several lots of fetal bovine serum were found to be adequate for supplementing the medium in the induction of F₁ hybrid anti-parent and anti-allogeneic cytotoxic effector cells.

Based on these and other studies, it would appear that the F₁ hybrid anti-parent cytotoxic response provides an in vitro model of murine hemopoietic graft rejection in vivo. This response may be elicited by a mechanism distinct from T cell-mediated cytotoxicity and involve different subpopulations of spleen cells.

Footnotes

¹ This work was supported in part by Grants (to G. C.) AM-13969 from the National Institute of Arthritis, Metabolism and Digestive Diseases, CA-12844 from the National Cancer Institute, and Contract NO1-CM-53766 from the Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, Md.