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The Kinetics of Interferon Production by Mouse Lymphocytes and Its Modulating Effect on the Virus Plaque-Forming Cell Assay As a Quantitative Method to Determine Activated Lymphocytes

Laboratory of Cellular and Comparative Physiology, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Public Health Service, United States Department of Health, Education and Welfare, Bethesda, and Baltimore City Hospitals, Baltimore, Maryland 21224

Abstract

The validity of the virus plaque-forming cell (V-PFC) assay as a means to quantitate stimulated mouse T cells is dependent on the rate and amount of coinciding interferon production which varies with different strains of mice.

In addition, the V-PFC assay demonstrates that the kinetics of cellular activation by mitogens initially involves about 0.01% of responding cells and possibly recruitment of other cells which include cells requiring a longer lag period for activation.