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Generation of Cytolytic T Lymphocytes in Vitro

VIII. Failure of anti-RS Antisera to Inhibit the Generation of Cytolytic T Lymphocytes or Cytolytic T Lymphocyte Activity¹

Swiss Institute for Experimental Cancer Research, The Department of Pathology, and the Franklin McLean Memorial Research Institute, The University of Chicago, ³ Chicago, Illinois, and the Department of Experimental Microbiology, Institute for Medical Microbiology, University of Zurich, CH-8006 Zurich, Switzerland

Abstract

Antibody reactive with "recognition structures" (RS) of mouse lymphoid cells for alloantigens (anti-RS) was prepared by immunization of F₁ hybrid mice with parental strain lymphoid cells or with antibody produced in one parental strain against alloantigens of the other parental strain. Such antisera prevented generation of the "product of antigenic recognition" (PAR) that is produced within a few hours in cultures prepared with a mixture of lymphoid cells from genetically disparate mice. However, treatment of responding lymphoid cells with anti-RS sera and complement did not inhibit generation of cytolytic T lymphocytes (CTL) in mixed lymphocyte cultures (MLC). Treatment of cells obtained from MLC with anti-RS sera and complement failed to inhibit cytolytic activity of such cells for specific alloantigens.

Footnotes

¹ Supported in part by National Institutes of Health Grants AI-4197 and AI-9268, and by grants from the Swiss National Foundation for Scientific Research.

² Fellow of the John Simon Guggenheim Memorial Foundation.

³ Operated by the University of Chicago for the United States Energy Research and Development Administration, under Contract No. E(11-1)-69.