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The Journal of Immunology, 1976, 117: 497-503.
Copyright © 1976 by The American Association of Immunologists, Inc.
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Rat T Lymphocyte-Specific Antigens and Their Cross-Reactivity with Mouse T Cells1

Yoshifumi Ishii, Hirofumi Koshiba, Hiroshi Yamaoka and Kokichi Kikuchi

Department of Pathology, Sapporo Medical College, Sapporo S1 W17, Hokkaido, Japan

Abstract

Anti-rat T lymphocyte serum (ATLS)2 was prepared by immunizing rabbits with purified T cells from rat mesenteric nodes and absorbed with rat red cells and syngeneic sarcoma cells. The specificity of ATLS for rat T cells was confirmed by the following reasons: a) ATLS was not toxic for bone marrow cells but lysed most of the thymocytes and a number of spleen and lymph node cells, which were inversely correlated to the percentage of cells with B cell characteristics in respective organs; b) anatomical localization of ATLS-reactive cells in lymphoid organs coincided to the thymus-dependent areas, i.e., the paracortex of lymph node and the periarteriolar region of spleen; c) spleen cells treated with ATLS and complement failed to respond to phytohemagglutinin but normally responded to bacterial lipopolysaccharide; d) those cells treated with ATLS and complement could not induce a graft-vs-host reaction in F1 hosts, whereas the same treatment did not affect direct plaque-forming cells. All of these data confirm the specificity of ATLS and indicate that ATLS recognizes rat T lymphocyte-specific antigens (RTLA). Absorption studies showed that RTLA were present in higher concentration on medullary thymocytes and peripheral T cells than on cortical thymocytes, but absent from bone marrow, liver, and brain tissues. When the cross-reactivity of RTLA with mouse T cells was studied by C-dependent cytotoxicity and immunofluorescence, it was found that mouse T cells shared at least one determinant of RTLA with rat T cells, and that distribution pattern of the cross-reacting antigens in mouse lymphoid tissues was essentially the same as that of RTLA in rat lymphoid organs.

Footnotes

1 This work was aided by a grant from the Ministry of Education, Government of Japan.

2 Abbreviations used in this paper: T, thymus-derived; B, bone marrow-derived; RTLA, rat T lymphocyte-specific antigens; PBS, phosphate-buffered saline; ALS, rabbit antiserum against rat lymph node T cells; ATLS, anti-rat T lymphocyte serum, C, complement, CI, cytotoxic index; Ig, immunoglobulins; GVH, graft-vs-host assay; PFC, plaque-forming cells; PHA, phytohemagglutinin, LPS, lipopolysaccharide; MPLA, antigens preferentially expressed on mature thymocytes and peripheral T cells in mcie.






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